Additional file 6: Figure S4.
Confirmation of further ORR1A0 splicing events in Klf3−/− erythroid cells that were not detected by RNA-Seq analysis. RNA from Klf3+/+ (WT), Klf3+/− (HET), and Klf3−/− (KO) TER119+ E14.5 fetal liver cells were analyzed by real-time RT-PCR using forward primers specific for ORR1A0 and reverse primers recognizing downstream exons of the Cd59b(A), Tmx4(B), and Bzw2, Cpe, and Tcfl5(C) genes. (A and B) Values have been normalized to 18S rRNA levels and WT samples have been set to 1.0. Error bars represent standard error of the mean (n = 2 WT, 3 HET, and 3 KO). *, P <0.05 (Student’s two-tailed t-test) compared to both Klf3+/+ and Klf3+/−. (C) For these genes, the spliced transcripts were below the level of detection in Klf3+/+ cells and thus could not be quantified. RT-PCR products were electrophoresed on a 3% agarose gel and stained with ethidium bromide.
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Mak et al. Genome Biology 2014 15:R58 doi:10.1186/gb-2014-15-4-r58