Additional file 3: Figure S3.
Pu.2 and ORR1A0 chimeric transcripts are induced rapidly by KLF1-ER and in the presence of cycloheximide. RNA from KLF1-ER inducible B1.6 erythroblast cells was analyzed by qRT-PCR using primers specific for Pu.2(A, B), ORR1A0-Znrf2(C, D), and ORR1A0-Brca2(E, F). In (A, C, and E), RNA was harvested at 0, 2, 4, 8, and 24 h following tamoxifen treatment (n = 2). In (B, D, and F), cells were treated with cycloheximide for 30 min prior to tamoxifen addition (or ethanol for untreated), with samples being taken 8 h thereafter (n = 4 for each condition). All values have been normalized to 18S rRNA levels and t = 0 h time points (A, C, and E) and tamoxifen-untreated samples (B, D, and F) have been set to 1.0. Error bars represent standard error of the mean. *, P <0.05 (Student’s one-tailed t-test) compared to t = 0 h (A, C, and E) and tamoxifen-untreated (B, D, and F). N.S, not significant.
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Mak et al. Genome Biology 2014 15:R58 doi:10.1186/gb-2014-15-4-r58