Common targets of SVP and AP1. (a) Expression analyses of ARF3, CLV1, KAN1, PHB, and PIN1. RNA was extracted from wild-type Col-0 and svp-41 agl24-2 ap1-12 triple mutant inflorescences. (b) Binding profiles of ChIP-seq experiment using inflorescence tissue for the selected genes. TAIR annotation corresponds to TAIR8. Black boxes represent the region validated by ChIP-PCR, which are shown in (c). (c) ChIP-PCR validation of selected genes using anti-GFP antibodies and inflorescences of wild-type Col-0 and the SVP::SVP-GFP svp-41 line. Error bars represent standard deviations (SD) of normalized data; (d) relative level of expression of ARF3, CLV1, KAN1, PHB, and PIN1 in svp-41 agl24-2 ap1-10, 35S: SVP-GR plants that were mock-treated or with 10 μM dexamethasone.
Gregis et al. Genome Biology 2013 14:R56 doi:10.1186/gb-2013-14-6-r56