Additional file 14.

Shotgun metagenomic sequencing validates predicted microbial metabolic trends in a subset of healthy and CD microbiomes. A subset of 11 stool samples for which microbial DNA was available were subjected to shallow metagenomic sequencing using the MiSeq platform (150-nucleotide paired-end reads) averaging 119 meganucleotides per sample. (a) Of the seven microbial metabolic modules highlighted in Figure 5, six retained the same over- or under-abundance trend predicted from 16S sequencing in this subset, with the seventh (cobalamin biosynthesis) falling below the limit of detection. (b) Six additional metabolic modules of interest with significant differences in the full IBD dataset retained the trend expected with CD in this subset, including depletion of glycolysis processes and enrichment for bacterial secretion systems.

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Morgan et al. Genome Biology 2012 13:R79   doi:10.1186/gb-2012-13-9-r79