Distribution and mean expression of 3SEQ peaks. (a) The distribution plot shows a tight cluster of exonic peaks approximately 275 bp upstream of the 3' end of known genes (n = 29,024 peaks in known exons; distances are based on genomic coordinates and not the spliced transcriptome). (b) Boxplots show the distribution of mean expression levels for each peak by peak category. Raw sequence count data was normalized by dividing each value by the sample mean, and then taking the square root. Boxes range from the first to the third quartiles. Median expression is marked with a line. Mean values are 0.799, 0.407 and 0.324 for coding, lncRNA and novel transcripts, respectively. Plots are truncated to show mean expression values less than 2. Outlier peaks show expression as high as 17.9.
Brunner et al. Genome Biology 2012 13:R75 doi:10.1186/gb-2012-13-8-r75