Additional file 6.

Morphology of tape-stripped skin and subsequent regeneration of human epidermis. (A) H&E staining of normal healthy skin. (B) The stratum corneum which is present in normal skin has been stripped off completely (biopsy taken 2 hours after tape-stripping). (C) A picture taken 4 hours after tape-stripping showing more hypertrophic basal cells, several pyknotic nuclei in cells of the stratum spinosum, and a layer of parakeratotic cells that begins to form on the surface. (D) At the stage of 24 hours after tape-stripping the basal cells are really hypertrophic and these columnar basal cells make up about one third of the thickness of the epidermis. Hyperparakeratosis is observed on top of the epidermis. (E) Pronounced acanthosis and hyperparakeratosis is seen after 48 hours. At this stage the tape-stripping skin model resembles most lesional psoriatic skin. (F) Finally at 96 hours, the hyperparakeratosis is disappeared and a fresh anuclear stratum corneum is formed on the emerging stratum granulosum. Scale bar = 100 μm.

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Zeeuwen et al. Genome Biology 2012 13:R101   doi:10.1186/gb-2012-13-11-r101