Involvement of Dicer partner proteins in the processing of various classes of small RNAs. (a) Schematic illustration of the substrates of Drosophila Dicer proteins and the products generated in the presence of different partner proteins. (b) Variability in 5' end processing is greater for miRNAs generated from the 3' arm of pre-miRNAs. The graph shows the cumulative distribution of the proportion of reads that correspond to the most abundant mature form in Ago2-CLIP (x-axis) for miRNAs generated from the 5' (black) and 3' (red) arm of the pre-miRNA. The vertical line corresponds to 90% of sequenced reads starting at the main processing site. cis-NAT, cis-natural antisense transcript; CLIP, UV cross-linking and immunoprecipitation; endo-siRNAs, endogenous siRNAs; exo-siRNAs, exogenous siRNAs.
Jaskiewicz and Zavolan Genome Biology 2012 13:179 doi:10.1186/gb-2012-13-11-179