Figure 2.

Separation of H4 isoforms according to degree of acetylation using WCX-HILIC. Isoforms with more acetylation carry less positive charges and, thus, have weaker electrostatic interaction with negative function groups (carboxylate for PolyC AT A) on the stationary phase and elute earlier.

Tian et al. Genome Biology 2012 13:R86   doi:10.1186/gb-2012-13-10-r86
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