Figure 2.

Simulations show TMM normalization is robust and outperforms library size normalization. (a) An example of the simulation results showing the need for normalization due to genes expressed uniquely in one sample (orange dots) and asymmetric DE (blue dots). (b) A lower false positive rate is observed using TMM normalization compared with standard normalization.

Robinson and Oshlack Genome Biology 2010 11:R25   doi:10.1186/gb-2010-11-3-r25
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