An example of ChIA-PET mapping display in G-browser. A screenshot of the ChIA-PET G-browser is shown in human chromosome 12 at the keratin gene family region. The tracks are (from top to bottom): UCSC known genes track, which shows the KRT8 and KRT18 genes in blue and green; density histogram of IHH015M self-ligation PETs, which consists of AA and BB PET sequences, wherein peaks indicate enriched ERα-binding sites; intra-chromosomal inter-ligation PETs of IHH015M, wherein multiple overlapping inter-ligation PETs indicate interactions and singleton inter-ligation PETs indicate noise; density histogram of IHH015C self-ligation PETs that consists of only chimeric (AB) PET sequences; intra-chromosomal inter-ligation PET tracks of IHH015C. From the graph, we know that IHH015M contains binding sites and interactions, and IHH015C does not, although both libraries have similar sequencing depth.
Li et al. Genome Biology 2010 11:R22 doi:10.1186/gb-2010-11-2-r22