Additional file 2.
High-resolution kinetics of response of genes to NO3- treatment. Levels of mRNA for nitrogen-responsive genes in Arabidopsis roots in response to NO3- treatment. Fourteen-day-old plants grown in the presence of ammonium succinate were treated with 1 mM KNO3 or KCL (as a mock treatment). Plants were collected at 0 minutes (before treatment) and 3, 6, 9, 12, 15, and 20 minutes after treatment. Transcripts were measured in RNA from roots using RT-QPCR and Affy ATH1 chips and normalized to two housekeeping genes (see Materials and methods). The data represent the mean ± standard error of three and two biological replicates for QPCR and Affymetrix measurements, respectively. Correlations between QPCR- and Affy-detected fold change results are provided in the third column.
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Krouk et al. Genome Biology 2010 11:R123 doi:10.1186/gb-2010-11-12-r123