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Two-hybrid assay in plants

Jonathan B Weitzman

Genome Biology 2001, 2:spotlight-20010807-01  doi:10.1186/gb-spotlight-20010807-01

The electronic version of this article is the complete one and can be found online at:

Published:7 August 2001

© 2001 BioMed Central Ltd

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In the August issue of Nature Biotechnology, Rajagopal Subramaniam and colleagues from the Université de Montreal describe a system for visualizing protein-protein interactions in living plant cells (Nature Biotechnology 2001, 19:769-772). Their technique is based on an in vivoprotein-fragment complementation assay in which protein interactions between fusion proteins induce folding and reassembly of fragments of the murine dihydrofolate reductase (DHFR) enzyme. Protein-protein interactions are monitored by the DHFR inhibitor fluorescein-conjugated methotrexate (fMTX). Subramaniam et al. used their assay to examine the interaction between Arabidopsis disease-resistance protein NPR1/NIM1 and the basic leucine-zipper protein TGA2. They show that salicylic acid induced NPR1-TGA2 interaction in tobacco or potato cells. This plant two-hybrid system should prove useful for the functional annotation of plant genomes.


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    Nature Biotechnology

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    Université de Montreal

  3. Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays.

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  4. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats

    PubMed Abstract | Publisher Full Text OpenURL