Additional file 1: Figure S1.
The chimeric Pu.1 transcript (Pu.2) is predominantly upregulated in erythroid tissues in the absence of KLF3. Total RNA was extracted from adult tissue from three Klf3+/+ (WT) and three Klf3−/− (KO) mice and analyzed by quantitative real-time RT-PCR using primers specific for the exon 2b/3 junction (A) or exon 2/3 junction (B) of Pu.1. Levels have been normalized to 18S rRNA and the lowest detectable reading in each chart has been set to 1.0. Error bars represent standard error of the mean. *, P <0.05 (Student’s two-tailed t-test) compared to wild-type.
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Mak et al. Genome Biology 2014 15:R58 doi:10.1186/gb-2014-15-4-r58