Transcripts containing exons towards the 3′ end of Pu.1 are upregulated in Klf3−/− E14.5 TER119+ fetal liver cells. (A, C, D) Transcript levels in Klf3+/+ (WT, n = 2), Klf3+/− (HET, n = 3), and Klf3−/− (KO, n = 3) cells were determined by quantitative real-time RT-PCR using forward and reverse primer combinations specific for exons 2 and 3 (A), exons 3 and 4 (C), or exons 4 and 5 (D) of Pu.1. Values have been normalized to 18S rRNA levels and in each instance the Klf3+/+ sample has been set to 1.0. Error bars represent standard error of the mean. *, P < 0.02 compared to both Klf3+/+ and Klf3+/− (Student’s two-tailed t-test). (B) Positions of microarray probes across the Pu.1 gene and their relative intensities in Klf3−/− compared to Klf3+/+ samples. Exons are displayed as blue boxes and are widened to denote the coding region. Schematic is not to scale.
Mak et al. Genome Biology 2014 15:R58 doi:10.1186/gb-2014-15-4-r58