Additional file 16.
Validation of the AS events in 19 genes by RT-PCR and corresponding IGV visualization. These 19 events include 9 alternative 5′SSs, 4 alternative 3′SSs and 6 exon-skipping events. For the validation of alternative 5′SSs and 3′SSs, there was only one band that represents the alternative-splice isoform, which was obviously detected in sad1 mutants, but not or only weakly detected in the wild type and SAD1-OE. For exon-skipping events, the alternatively spliced forms were marked with grey asterisks (*). For IGV visualization, alternative splicing sites were marked by red arrows and highlighted by red arcs.
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Cui et al. Genome Biology 2014 15:R1 doi:10.1186/gb-2014-15-1-r1