Differential expression analysis using qRT-PCR validated gene set. (a) ROC analysis was performed using a qRT-PCR log2 expression change threshold of 0.5. The results show a slight advantage for DESeq and edgeR in detection accuracy. (b) At increasing log2 expression ratios (incremented by 0.1), representing a more stringent cutoff for differential expression, the performances of the Cuffdiff and limma methods gradually reduce whereas PoissonSeq performance increases. AUC, area under the curve.
Rapaport et al. Genome Biology 2013 14:R95 doi:10.1186/gb-2013-14-9-r95