Analysis of E. invadens introns. (a) Sequence logos representing consensus sequences at 5' and 3' intron-exon junctions for introns detected by whole transcriptome mapping (upper) and for introns predicted by computational gene prediction but not confirmed by transcriptome mapping (lower). The start and end of the intron are indicated by the thick black line and intronic sequence by the thin line. The dashed line indicates that the central portions of the introns were not analyzed. (b) Example of a probably incorrect gene model (EIN_014820, thick black line) that contains an intron (thin black line) that is contradicted by transcriptome mapping (depth of coverage shown in grey plot). (c) Example of probably incorrect gene model (EIN_035150, part shown with predicted translation) in which the predicted intron (dashed line) is supported by whole transcriptome mapping at the 5' junction only. The correct intron is indicated by the solid black line. The corrected protein is shorter by four amino acids (TDET).
Ehrenkaufer et al. Genome Biology 2013 14:R77 doi:10.1186/gb-2013-14-7-r77