So, you want to sequence a genome...
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
Genome Biology 2013, 14:128 doi:10.1186/gb-2013-14-7-128Published: 30 July 2013
First paragraph (this article has no abstract)
Anyone who has attempted to identify the responsible gene or mutation underlying a disease or mutant phenotype will know the importance of an accurate reference genome assembly. For complex vertebrate genomes, however, generating such an assembly is not trivial, even with new sequencing technologies. In 2007, a mid-point in the zebrafish genome sequencing project, I was asked to lead the project to completion. At that point we were faced with a highly fragmented physical assembly and lacked genetic maps of sufficient density and resolution to produce an accurate assembly.