Maintenance and loss of H3K27me3 marks 10 days after priming. Average relative amounts of DNA amplified by qPCR from anti-H3K27me3 ChIP samples obtained from roots of primed (P, dark color) and non-primed (C, light colors) plants immediately after the 24-h priming treatment (24 h, blue) or 10 days later (10 d, turquoise). Each value was normalized to ChIP input and to constitutive reference region in At5g56920. Bars are means ± SE of three independently treated replicate plant batches each consisting of approximately 300 plants (same material as pooled for ChIP-sequencing). Significant differences between primed and non-primed plants are indicated with * for P <0.05 and ** for P <0.01.
Sani et al. Genome Biology 2013 14:R59 doi:10.1186/gb-2013-14-6-r59