Transcript and H3K27me3 profiles of HKT1 in primed and non-primed plants. (A) mRNA levels of HKT1 (relative to constitutive gene RpII) determined by qPCR in roots of primed plants (PR, light grey and black bars) or non-primed plants (CR, white and dark grey bars) 10 days after priming and 4 h after application of 0 (-, control) or 80 mM NaCl (+, stress treatment). Inset shows very low expression of HKT1 in the shoots of the same plants. Results are shown separately for three independently primed and treated plant batches (Rep1-3) each consisting of pooled tissue from 12 plants. Bars are means ± SE of four pairwise ratios of two technical replicates. Significant differences between primed and non-primed plants for each condition (+/- salt) are indicated with * for P <0.05 or ** for P <0.01. (B) H3K27me3 profile over the HKT1 sequence in primed (PR) and non-primed (CR) root immediately after priming (24h) and 10 days later (10d) as displayed in IGB. The differential site identified by CHIPDIFF in the 24-h samples is marked with a white box.
Sani et al. Genome Biology 2013 14:R59 doi:10.1186/gb-2013-14-6-r59