Additional file 15.

Asynchronous and mitotic GFP-tagged HeLa cells. Green fluorescent protein appears in green and cell boundaries in orange. (A) In an asynchronous cell culture only a few cells are in the mitotic phase, which can be seen from the condensed chromatin and the rounded cell morphology. (B) Cell obtained with mitotic shake-off. The procedure enriches for round cells containing condensed chromatin.

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Kishore et al. Genome Biology 2013 14:R45   doi:10.1186/gb-2013-14-5-r45