Reversibility of chromosome repositioning effects. (a) Wild-type HT1080 cells were separately treated with small interfering RNA (siRNA) oligos to deplete NET29 or NET39. Western blots of control siRNA and targeting siRNA-treated cells are shown. The signal intensity of bands on the blots was quantified and the band intensity for each siRNA was set to 1 for day 0. The normalized intensity of the bands over the time course is shown above each band. (b) Chromosome 13 position was quantified. Depletion of either NET reduced the endogenous peripheral positioning of chromosome 13. Error bars indicate standard deviation between the means of individual experiments. *P < 0.05 and **P < 0.01, comparing the position of the chromosome in the si NET cells to the si scramble control using KS tests. Statistics are given in Additional file 1.
Zuleger et al. Genome Biology 2013 14:R14 doi:10.1186/gb-2013-14-2-r14