Additional file 3.

Computational identification of crosslinking sites. (A) Illustration of sequence block generation, Gaussian distribution fitting, and cluster segmentation to identify individual crosslinking sites. (B) Pearson correlation coefficients for all gPAR-CLIP and mRNA-seq replicate libraries based on gene RPM values. (C) Separation of T-to-C sequencing errors from crosslinking-induced mismatches. Plotted for each cluster is T-to-C RPM coverage versus total RPM coverage from gPAR-CLIP or mRNA-seq libraries. (D) Percentage of annotated 5' UTR, CDS, and 3' UTR regions with at least one crosslinking site with >5 RPM.

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Freeberg et al. Genome Biology 2013 14:R13   doi:10.1186/gb-2013-14-2-r13