Additional file 13.
Intra-replicate variation of crosslinking site coverage and global changes in 5' UTR crosslinking sites. (A) Determination of minimum crosslinking site coverage required for comparison of sites across environmental conditions. We chose 5 RPM as the minimum crosslinking site coverage needed for confident quantification since, at this coverage, the standard deviation of the fraction of crosslinking site reads coming from one replicate library stabilized at <0.2. Shown are data from wild-type (WT) replicate libraries; similar results were obtained for all library types. (B) Intra-replicate variation of 3' UTR crosslinking sites in WT and glucose (left) or nitrogen (right) starvation conditions. Dotted lines represent three standard deviations from the mean and correspond to approximately four-fold change between WT replicates. (C) Same as (A) but for 5' UTR crosslinking sites. (D) Global changes in 5' UTR crosslinking site coverage upon glucose (left) or nitrogen (right) starvation.
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Freeberg et al. Genome Biology 2013 14:R13 doi:10.1186/gb-2013-14-2-r13