Additional file 1.

Tables S1 to S3 and figures S1 to S3. Table S1: synthetic miRNA library. Complete list of 29 synthetic miRNAs and the primary sequences used in miRNA-Seq studies. Table S2: GC content of synthetic miRNA library. List of 29 synthetic miRNAs used in this study, their corresponding GC content listed as a percentage of total nucleotide number, and their observed cloning frequency listed as a percentage of total mapped miRNA reads. Table S3: oligonucleotides used in this study. List of oligonucleotides used in the ligation optimization and miRNA-Seq experiments. Figure S1: clustered miRNAs 143/145 exhibit differential quantification in a method-specific manner. (A) miRNA-Seq from mouse hair follicle. (B) qRT-PCR quantification of mouse epidermis determined by ΔΔCt method where sno25 serves as the reference gene. Figure S2: DNA oligos can be readily adenylated by MTH (Methanobacterium thermoautotrophicum) RNA ligase. 18% urea-PAGE of 3′ DNA linkers shows shift in electrophoretic mobility corresponding to 5′ adenylation where plus and minus signs indicate the presence or lack of MTH enzyme, respectively. Figure S3: enhanced miRNA-Seq approach is highly correlative for varying amounts of input RNA. Differing amounts of the 29 synthetic miRNA mix were subjected to enhanced miRNA-Seq.

Format: PDF Size: 422KB Download file

This file can be viewed with: Adobe Acrobat Reader

Zhang et al. Genome Biology 2013 14:R109   doi:10.1186/gb-2013-14-10-r109