Characterization of chromatin in the vicinity of Dxz4. (a) Schematic map of the region encompassing Dxz4 and the downstream satellite repeat (diverging open arrows). Left-pointing facing arrows represent Dxz4, and the location of the CGI and promoters for Dxz4 and Pls3 are indicated. The angled double strike through the map between the Dxz4 and Pls3 promoters represents an approximately 114-kb gap. The red boxes indicate the regions assessed by chromatin immunoprecipitation (ChIP)-PCR. (b) Graphs showing results of ChIP assayed by quantitative PCR. The mean and standard deviation for the ChIP elution (IP) and for a negative control rabbit serum (RS) are shown as percentage of the input. For H3K4me2 and H3K27me3 at the Dxz4 promoter (Dxz4-Prom) and Pls3 promoter (Pls3-Prom), data for one male and one female are shown. For H3K4me2 and Ctcf at Dxz4, DS-TR and DD-CGI, data are shown for two independent male and female samples. (c) Pie charts showing the percentage of C57BL/6J (B6) or castaneous (Cast) informative allele calls for Ctcf ChIP-Seq fragments for Dxz4 and the downstream tandem repeat (Ds-TR) Ctcf binding sites.
Horakova et al. Genome Biology 2012 13:R70 doi:10.1186/gb-2012-13-8-r70