Isolation of Brd- and HP1-bound mononucleosomes. (a) Illustration of the domain architecture of the histone code readers. (b) Schematic of protocol used to isolate nucleosomes bound to FLAG-histone code readers (HCRs). (c) Visualization of FLAG-Brd- and FLAG-HP1-bound nucleosome purifications. The proteins present in the input (I), the immunoprecipitation unbound (U), and FLAG-peptide-eluted (E), fractions were examined by SDS-PAGE and Coomassie blue staining. The asterisk denotes Brd3 breakdown products detected by liquid chromatography-tandem mass spectrometry.
LeRoy et al. Genome Biology 2012 13:R68 doi:10.1186/gb-2012-13-8-r68