The amplitude and phase of circadian exons is tissue-dependent. (a) Plots of Pcsk4 alternative splicing (left panel) and transcript-level expression (right panel, GE, gene expression) through time in liver (pink), kidney (blue) and lung (green). In each case, n = 6 and standard error of the mean. Asterisks indicate results of a one-way ANOVA for an effect of time (**P ≤ 0.01, ***P ≤ 0.001). Individual tissue time-courses were examined where a two-way ANOVA had revealed a significant (P ≤ 0.05) main effect of tissue and/or an interaction effect between tissue and time. (b) Summary of alternative splicing data from the other validated exons (Figure S4 in Additional file 1). Left panel: acrophase of alternative splicing (AS) in three tissues. Acrophase was defined as the circadian time of the largest positive fold change versus CT0. Right panel: amplitude of alternative splicing in three tissues. Amplitude was defined as the biggest absolute value of fold change versus CT0 for exon-specific QPCR.
McGlincy et al. Genome Biology 2012 13:R54 doi:10.1186/gb-2012-13-6-r54