FIRMA analysis of exon-arrays reveals circadian alternative splicing in mouse liver. (a) Representative double-plotted actogram showing the circadian locomotor activity of the wild-type mice used in this study. Vertical black lines represent infrared beam brakes, which reflect locomotor activity. LD, 12 h light followed by 12 h dark-red light; DD, constant dark-red light. The white and black boxes atop the actogram represent the lighting schedule (LD)/subjective day and night (DD). Descending blue lines indicate the times at which samples were harvested. (b) Circadian FIRMA score profiles were clustered into four groups using a self-organizing tree algorithm . For each panel, grey lines represent the exon-probesets comprising that cluster, whereas the red line represents the cluster centroid. Cluster 1, top left; cluster 2, top right; cluster 3, bottom left; cluster 4, bottom right (Additional file 2).
McGlincy et al. Genome Biology 2012 13:R54 doi:10.1186/gb-2012-13-6-r54