Duplication of the Layn gene locus in SHR results in transcription from the original and the extra allele. (a) Genomic coverage (averaged over 999 bp) in SHR at the Mx2 locus. The site of the duplication is indicated with the white bar. (b) Expression levels as measured by normalized RNA-seq read counts in the coding regions of the MX2 gene. The asterisk indicates significant difference (FDR <0.05). (c) Eight heterozygous SNVs were found in the exons of Layn in the genomic sequencing data of SHR. RNA-seq reads covering these positions were analyzed to investigate if they contained the reference or the alternative allele.
Simonis et al. Genome Biology 2012 13:r31 doi:10.1186/gb-2012-13-4-r31