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The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome

R Kimberley Cook, Stacey J Christensen, Jennifer A Deal, Rachel A Coburn, Megan E Deal, Jill M Gresens, Thomas C Kaufman and Kevin R Cook*

Author Affiliations

Bloomington Drosophila Stock Center, Department of Biology, Indiana University, 1001 E. Third St, Bloomington, IN 47405, USA

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Genome Biology 2012, 13:R21  doi:10.1186/gb-2012-13-3-r21

Published: 22 March 2012

Additional files

Additional file 1:

Breakpoints and characterization of BSC deletions. This file provides full information for deletions isolated in this project, including progenitor FRT insertions and their locations, selection criteria in screens, verification tests and references to FlyBase documentation.

Format: XLSX Size: 170KB Download file

Open Data

Additional file 2:

Map of X and fourth chromosome deletion breakpoints. This spreadsheet shows the breakpoints and progenitor FRT insertions of Exelixis, DrosDel and BSC deletions positioned relative to the proximal and distal ends of annotated genes. It also shows the breakpoints of other deletions in the BDSC Deficiency Kit. The filled-in cells in columns to the right depict deletions graphically. Similarly, the positions of haploinsufficient genes are highlighted in colored rows to show their relationships to the deletions. Notes explain how we estimated the positions of breakpoints for deletions in the Deficiency Kit that have not been molecularly characterized.

Format: XLSX Size: 7.5MB Download file

Open Data

Additional file 3:

Haploinsufficient genes with viability and fertility effects. This table gives details of the mapping and identification of haplolethal and haplosterile genes in D. melanogaster, including cytological locations, hemizygous phenotypes, deletions removing the genes, deletions most closely flanking the genes and the number of closely linked genes sharing the same deletion breakpoint interval.

Format: XLSX Size: 42KB Download file

Open Data

Additional file 4:

Bloomington Drosophila Stock Center Deficiency Kit stocks. This file lists the component deletions in the BDSC Deficiency Kit with their breakpoints and stock information.

Format: XLSX Size: 47KB Download file

Open Data