Biogenesis of nat-siRNAs that depend on DCL3. (a-c) Accumulation of nat-siRNA1g13340 (a), nat-siRNA1g28100 (b) and nat-siRNA1g17745 (c)Total RNA (75 to 100 μg) of distinct dcl, rdr, polIV and polV mutants and corresponding WT are probed by LNA probes indicated in Figures 1 and 3. U6 was used as a loading control. Similar results were obtained in two biological repeats.
Zhang et al. Genome Biology 2012 13:R20 doi:10.1186/gb-2012-13-3-r20