Number of reads mapped to B. pertussis. Number of mapped reads and base substitution errors in 4.2 million reads of 76 cycle paired-end B. pertussis data relative to a reference genome. The base callers Bustard, Ibis and AYB are compared on ends 1 and 2 of the reads by the number of differences to the reference, to a maximum of five differences, with the total percentage of mapped reads displayed at the top of each bar. Results for both the full reads (left, 'All 76 cycles') and reads trimmed to the first 50 cycles (right, 'First 50 cycles') are shown.
Massingham and Goldman Genome Biology 2012 13:R13 doi:10.1186/gb-2012-13-2-r13