Classification of Ler-specific H3K27me3 targets based on their expression. (a) Expression pattern of Ler-specific H3K27me3 targets in Col in different tissues. The expression value was normalized to the mean of all genes within each sample. Five expressed Ler-specific targets (Exp_Col) are framed in red. The developmental stages and tissues used for experiments are: roots at 7 days (Ro); seedlings, aerial parts, 7 days (Se7); expanding leaves, 10 days (Le10); senescing leaves, 35 days (Le35); stem, 2nd internode (St); vegetative shoot meristem, 7 days (Me7); inflorescence shoot meristem, 21 days (Inf21); whole inflorescences to floral stage 9, 21 days (Me21); whole inflorescences of clavata3-7 mutants, 21 days (clv); flowers, stage 15, 21+ days (Fl21); fruits, carpels stage 15, 21+ days (Fr21). The data were obtained from the Arabidopsis thaliana Tiling Array Express (At-TAX) resource . (b) Expression pattern of Ler-specific targets in 19 Arabidopsis accessions. Accessions indicated below the heatmap are Columbia-0 (Col), Catania-1 (Ct), Tsushima-0 (Tsu), Kaunas-0 (Kn), Würzburg-0 (Wu), Landsberg erecta (Ler), Burren-0 (Bur), Martuba-0 (Mt), Nossen-0 (No), Hilversum-0 (Hi), Rschew-4 (Rsc), Canary Islands-0 (Can), Oystese-0 (Oy), Wilna-2 (Wil), Poppelsdorf-0 (Po), Edinburgh-0 (Edi), Wassilewskija-0 (Ws), San Feli-2 (Sf), Zurich-0 (Zu). AT2G20910 (arrow) did not express in Col but in Ler and another three accessions. Gray indicates that the genes were not analyzable in the data-set, which was generated by the 19 Genomes Project . RPKM, reads per kilobase per million mapped reads. (c) Expression of nine Ler-specific H3K27me3 targets in Col and Ler at different developmental stages or tissues. Expression was measured by qRT-PCR, and is plotted relative to the reference gene AT1G13320. Analyzed time points and tissues are as indicated. Expression was measured in two independent experiments with similar results.
Dong et al. Genome Biology 2012 13:R117 doi:10.1186/gb-2012-13-12-r117