FOCAL refinement cycle. The model testing and refinement cycle is a three part process. First, FOCAL is used to design experiments where a particular reaction should be essential. The necessary mutants and media are prepared and growth phenotype experiments are performed. If any discrepancies are observed, the errors are corrected using various methods to suggest model improvements. These modifications can subsequently be tested further by designing new FOCAL designed experiments based on the refined model.
Tervo and Reed Genome Biology 2012 13:R116 doi:10.1186/gb-2012-13-12-r116