Effects of fluperlapine on Chlamydomonas. (a-c) Dibenzazapine analogs: (a) fluperlapine; (b) clozapine; (c) clothiapine. Common core structure is shown in black, variable regions in red. (d) Chlamydomonas cells grown in TAP with 0.5% DMSO. (e) Cells grown in TAP with 25 µM fluperlapine. (f) Fluperlapine-induced cell cluster produces four distinct flagella-less cells upon applied pressure. Parental wall is outlined in a dashed white line. (g) Flagellar regeneration of Chlamydomonas in solvent (closed squares) or 100 µM fluperlapine (open squares). Length of at least 20 flagella per time point, averaged from two independent replicates is plotted. Error bars represent standard error. Scale bar 10 µm.
Alfred et al. Genome Biology 2012 13:R105 doi:10.1186/gb-2012-13-11-r105