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A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis

Simon E Alfred123, Anuradha Surendra124, Chris Le1, Ken Lin1, Alexander Mok1, Iain M Wallace16, Michael Proctor5, Malene L Urbanus14, Guri Giaever134 and Corey Nislow123*

Author Affiliations

1 Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada

2 Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada

3 Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5A 1A8, Canada

4 Department of Pharmaceutical Sciences, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada

5 Stanford Genome Technology Center, Palo Alto, CA 94304, USA

6 Novartis, 250 Massachusetts Ave., Cambridge 02139, USA

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Genome Biology 2012, 13:R105  doi:10.1186/gb-2012-13-11-r105

Published: 18 November 2012

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Additional file 1:

Supplemental Figures S1, S2, S3, and S4.

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Additional file 2:

Supplemental Tables S1 and S2. Proschold T: Portrait of a Species: Chlamydomonas reinhardtii. Genetics 2005, 170:1601-1610. Ehler L, Holmes J: Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics 1995, 141:945-960. Goodenough UW, StClair HS: BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii. Journal of Cell Biology 1975, 66:480-491. Luck D, Piperno G, Ramanis Z, Huang B: Flagellar mutants of Chlamydomonas: studies of radial spoke-defective strains by dikaryon and revertant analysis. Proceedings of the National Academy of Sciences of the United States of America 1977, 74:3456-3460. Piperno G, Huang B, Luck DJ: Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences of the United States of America 1977, 74:1600-1604. Galloway RE, Mets L: Non-Mendelian inheritance of 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea-resistant thylakoid membrane properties in Chlamydomonas. Plant Physiology 1982, 70:1673.

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