Normalized per-base sequencing-depth distribution on targets. For the purpose of comparison among the three platforms, we selected a set of reads with an average coverage of approximately 30-fold from each replicate. The depth and the frequency (the fraction of a certain depth-level bases for certain sequencing depth-coverage in the total sequencing data) were normalized by the average coverage depth of each replicate on targets. NA-r1 and NA-r2, NS-r1 and NS-r2, and AS-r1 and AS-r2 represent each of two replicates for NimbleGen Sequence Capture Arrays, NimbleGen SeqCap EZ and Agilent SureSelect, respectively.
Asan et al. Genome Biology 2011 12:R95 doi:10.1186/gb-2011-12-9-r95