Additional file 4.

Influence of bait properties on capture efficiency. MDC was calculated for 47,874 2× tiled baits. All baits with a MDC above 200 were removed from the analysis. MDC was plotted against different bait parameters values: GC, bait GC content; k-mers, median frequency of the bait sequence in the Chinese spring genome; PMFE, probe minimum folding energy; PHFE, probe hybridization free energy; HS, bait Hairpin score; DS, bait Dimer score; Tm1 and Tm2, melting temperatures 1 and 2. Details of parameter estimations are provided in the Materials and methods. Red curves represent the median of MDC per value of a parameter.

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Saintenac et al. Genome Biology 2011 12:R88   doi:10.1186/gb-2011-12-9-r88