Additional file 5.

Experimental validation of detected transcripts by RT-PCR. (a) Agarose gel electrophoresis of amplicons amplified using primers designed against 14 different sequences from the Illumina+ assembly and two control sequences. (b) Primer sequences used in the PCRs.

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Sandmann et al. Genome Biology 2011 12:R76   doi:10.1186/gb-2011-12-8-r76