Additional file 4.

Experimental validation of the continuity of genomic supercontigs v31.005068 and v31.000152. (a) Agarose gel electrophoresis showing the PCR amplicon produced with forward and reverse primers annealing to supercontigs v31.005068 and v31.000152, respectively. (b) The amplicon sequence was verified by Sanger sequencing. (c) Blastn results showing the 5' end of the sequence aligning to supercontig v31.000152 and the 3' end matching supercontig v31.005068.

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Sandmann et al. Genome Biology 2011 12:R76   doi:10.1186/gb-2011-12-8-r76