Positions of chromosomes 10, 18 and × in HGPS human dermal fibroblasts following serum starvation Proliferating control and HGPS (AG11498) cells were placed in low serum (0.5%) for 5 minutes, 10 minutes, 15 minutes, 30 minutes and 7 days. The cells were subjected to two-dimensional FISH and the nuclear location of chromosome 10 (I:a-f, II:a-f,), chromosome 18 (I:g-l, II:g-l,) and the X chromosome (I:m-r, II:m-r,) assessed by erosion analysis. Unpaired, unequal variances two-tailed students t-tests were performed to assess statistical differences between the fibroblasts at 0 minutes and the cells placed in low serum. The filled-in squares represent a significant difference (P < 0.05). Panel I demonstrates that when control fibroblasts are placed in low serum, chromosome 10 moves from an intermediate position to a peripheral position (I:a-f), chromosome 18 moves from a peripheral position to an interior location (I:g-l) and chromosome × remains at the nuclear periphery (I:m-r). These changes in position have been revealed previously [40,42].
Mehta et al. Genome Biology 2011 12:R74 doi:10.1186/gb-2011-12-8-r74