Contribution of different factors to the overall cost of a sequencing project across time. Left, the four-step process: (i) experimental design and sample collection, (ii) sequencing, (iii) data reduction and management, and (iv) downstream analysis. Right, the changes over time of relative impact of these four components of a sequencing experiment. BAM, Binary Sequence Alignment/Map; BED, Browser Extensible Data; CRAM, compression algorithm; MRF, Mapped Read Format; NGS, next-generation sequencing; TAR, transcriptionally active region; VCF, Variant Call Format.
Sboner et al. Genome Biology 2011 12:125 doi:10.1186/gb-2011-12-8-125