Average induction of the 32 inducible clones by q-PCR. Baseline expression (0 hours of induction - white bars), following induction of transgene (after 24 to 48 hours of growth in medium deprived of Tc - gray bars), and relative expression in the parental cell line (EB3 - black bars). (a) The 13 transcription factors and the single transcriptional activator (Dscr1-Rcan1); (b) the 6 kinases; (c) the other 12 genes with diverse molecular functions. Asterisks indicate statistically significant expression changes (t-test with false discovery rate <0.05). The errors bars are calculated on the biological triplicates.
De Cegli et al. Genome Biology 2010 11:R64 doi:10.1186/gb-2010-11-6-r64