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Active DNA demethylation in human postmitotic cells correlates with activating histone modifications, but not transcription levels

Maja Klug1, Sven Heinz2, Claudia Gebhard1, Lucia Schwarzfischer1, Stefan W Krause3, Reinhard Andreesen1 and Michael Rehli1*

Author Affiliations

1 Department of Hematology, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93042 Regensburg, Germany

2 Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA

3 Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Krankenhausstraße 12, 91054 Erlangen, Germany

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Genome Biology 2010, 11:R63  doi:10.1186/gb-2010-11-6-r63

Published: 18 June 2010

Additional files

Additional file 1:

Supplementary methods, additional results, description of supplementary tables, and supplementary figures.

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Additional file 2:

Oligonucleotides for bisulfite amplicon generation. Genomic locations and oligonucleotides for EpiTYPER bisulfite amplicons.

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Additional file 3:

MassARRAY EpiTYPER results. EpiTYPER methylation ratios of individual CpG units in 46 amplicons covering 26 distinct genomic locations are given for all samples of different donors along with mean values for d7 macrophages (MAC), monocytes (MO), dendritic cells (DC) at day 7 or 51 h and data for unmethylated, 33%, 66% and 100% methylated control DNA. Amplicons were grouped according to their microarray results: MCIp different (regions were detected as differentially methylated between MAC and DC; 18 regions in total, three are marked as false positive in the microarray experiments), MCIp marginally different (one region), MCIp ND (one region - CCL17 promoter - that was not present on the array but represented a possible target; MCIp control (regions that were detected as equally methylated (or unmethylated) between MACs and DCs, 6 regions). For two additional regions (HLA-DPB/A1 and SLC7A8), none of the tested amplicons worked.

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Additional file 4:

Oligonucleotide sequences used for quantitative PCR, cloning, and electrophoretic mobility shift assay.

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