True positive SNVs identified as a function of the amount of sequence data generated. The number of true positive SNVs identified by RNA-Seq is shown for between one and eight lanes of sequence data, for exonic, core exonic and PBMC-expressed SNVs. PBMC-expressed genes are designated as those with an expression level of at least 4% of the most highly expressed PBMC transcript. There were approximately 35 million sequence reads in each lane.
Cirulli et al. Genome Biology 2010 11:R57 doi:10.1186/gb-2010-11-5-r57