Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
1 Department of Biology, Stanford University, Hopkins Marine Station, Oceanview Blvd Pacific Grove, CA 93950, USA
2 Current address: Department of Biology, 107 Life Sciences Complex, Syracuse University, Syracuse, NY 13244, USA
Genome Biology 2010, 11:R44 doi:10.1186/gb-2010-11-4-r44Published: 19 April 2010
High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance microarray-based genotyping for population genomics with Restriction Site Tiling Analysis. The approach simultaneously discovers polymorphisms and provides quantitative genotype data at 10,000s of loci. It is highly accurate and free from ascertainment bias. We apply the approach to uncover genomic differentiation in the purple sea urchin.