Additional file 5.

SPL9 over-expression modifies NO3- kinetic responses of transcription factors. Transcription factor mRNA levels in roots of wild type and transgenic pSPL9:rSPL9 in response to NO3- treatment. Fourteen-day-old plants grown in ammonium succinate were treated with 1 mM KNO3 versus KCl. Roots were collected at 0 minutes (before treatment) and 10, 20, 40, and 60 minutes after the treatment. Sentinel transcripts were measured in roots using RT-QPCR and normalized to two housekeeping genes (see Materials and methods). The data represent the mean ± standard error of three biological replicates (three independent experiments). Differences between the two genotypes are statistically significant at *P < 0.05, **P < 0.01, and ***P < 0.001 (t-test).

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Krouk et al. Genome Biology 2010 11:R123   doi:10.1186/gb-2010-11-12-r123