PCR-free reduction in G+C coverage bias and direct-from-colony coverage distribution. (a) Coverage with respect to G+C content in E. coli with and without PCR was assessed by calculating G+C content of the reference in 500 bp bins and plotting the coverage in each for transposase after PCR (left) and with the PCR-free method (right). A significant reduction in coverage bias at the extremes of G+C content is observed. (b) Coverage distribution for E. coli library prepared directly from cell lysate without purification.
Adey et al. Genome Biology 2010 11:R119 doi:10.1186/gb-2010-11-12-r119