De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data
- Equal contributors
1 Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
2 BC Cancer Agency Genome Sciences Centre, Vancouver, BC, V5Z 4E6, Canada
3 Amyris Biotechnologies, Inc., Hollis Street, Emeryville, CA 94608, USA
4 Washington University School of Medicine, Forest Park Ave, St Louis, MO 63108, USA
5 Natural Resources Canada, rue du PEPS, Ste-Foy, Quebec, G1V 4C7, Canada
6 Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
Genome Biology 2009, 10:R94 doi:10.1186/gb-2009-10-9-r94Published: 11 September 2009
Additional data file 1:
Supplementary sections 1: additional explanation for the 454 read filtering and alignments of the 454 pre- and post- filtered Forge assemblies relative to the manually finished GCgb1 sequence. Supplementary sections 2: additional explanation and supporting figures for the filtering and trimming of Illumina PE read data. Supplementary sections 3: supporting Figure S5 detailing the read coverage in the final assembly as well as preliminary repeat annotations and highlighting a small number of misassemblies identified with Illumina PE alignment clusters.
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Additional data file 2:
NCBI accession numbers for the trace data used in this study.
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